纳米炭在乳腺癌前哨淋巴结示踪的实验研究
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张亚男E-mail:zhangyanan79@126.com

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R 737.9

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江苏省科技厅社会发展资助项目(BS2006046)。


ExpExperimental study on sentinel lymph node biopsy of breast cancer by carbon nanoparticles suspension injection
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    摘要:

    目的:比较和评价纳米炭混悬注射液(卡纳琳)及亚甲蓝注射液进行乳癌淋巴结示踪的作用差别,为临床乳腺癌患者的治疗提供相关手段。
    方法:取40只雌性成年大耳兔,将VX2细胞株制成的瘤组织块悬液注射于兔乳腺下方制成乳腺癌模型,并将成癌模型分成卡纳琳组及亚甲蓝组,分别行淋巴结活检。记录第一枚淋巴结成功染色枚数、染色所需时间、褪色所需时间、染色淋巴结总枚数,并观察染色效果。
    结果:两组第1枚淋巴结染色成功率相同,所需时间无统计学差异;卡纳琳组淋巴结染色总枚数明显多于亚甲蓝组,差异有统计学意义(P<0.05);卡纳琳组褪色时间明显慢于亚甲蓝组,且染色效果优于亚甲蓝。
    结论:用VX2细胞株制成的瘤组织块悬液注射于兔乳腺下方可成功制成理想的兔转移性乳腺癌模型。纳米炭混悬液行淋巴结示踪染色效果优于亚甲蓝,为更理想的乳腺癌手术淋巴示踪剂。

    Abstract:

    Objective:To compare the efficacy of carbon nanoparticles suspension and methylene blue for the detection of axillary sentinel lymph nodes in rabbits with breast cancer, to provide a method for the clinical application of treatment of breast cancer.
    Methods:The model of breast cancer was established in 40 rabbits by intramammary injection of VX2 tumor tissue suspension 1ml. The successful model was divided into two groups. Carbon nanoparticles suspension and methylene blue were used in staining sentinel lymph node. The staining time, stain fading time of SLN, number of stained SLN, total number of the stained lymph node and the staining effect were observed.
    Results:The staining time of SLN and number of stained SLN had no significant difference between the 2 groups, total number of the stained lymph nodes was 3.25±0.56 and 2.3±0.21 respectively in carbon nanoparticles suspension group and methylene blue group(t=6.99, P< 0.05), while the stain fadeing time was more than 180 min in carbon nanoparticles suspension group and 105.75 min(average) in methylene blue group. The staining effect in carbon nanoparticles suspension group was much better than that in the methylene blue group.
    Conclusions:This study confirms that the rabbit metastatic model of breast cancer set up by intramammary injection of VX2 tumor tissue suspension is an ideal model. Carbon nanoparticles suspension is superior to methylene blue for sentinel lymph node staining, and is a more ideal lymphatic tracer for breast cancer cancer surgery.

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瞿欢|张亚男|陈卫东.纳米炭在乳腺癌前哨淋巴结示踪的实验研究[J].中国普通外科杂志,2010,19(5):489-492.

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  • 收稿日期:2010-01-07
  • 最后修改日期:2010-03-14
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  • 在线发布日期: 2010-05-15
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