MAP3K10在胰腺癌中的表达及其意义
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秦仁义 E-mail:ryqin@tjh.tjmu.edu.cn

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R 735.9

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国家自然科学基金资助项目(81071775)


Expression and significance of MAP3K10 in pancreatic carcinoma  
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    摘要:

    目的:探讨丝裂原活化蛋白3激酶10(MAP3K10)在胰腺癌中的表达。
    方法:应用免疫组织化学染色技术,Western blot及定量RT-PCR检测MAP3K10在胰腺癌及癌旁组织中的表达。
    结果:(1)免疫组化染色阳性细胞计数显示MAP3K10在胰腺癌组织中的阳性细胞数(100%呈强阳性)明显多于癌旁组织(仅25%呈弱阳性)(P<0.05);(2)Western blot检测显示,在91.7%的胰腺癌组织中MPA3K10蛋白的表达量高于癌旁组织;(3)qRT-PCR检测显示,MAP3K10mRNA在胰腺癌及癌旁组织中均有表达,但前者的表达水平明显高于后者(P<0.01)。
    结论:MAP3K10在胰腺癌中存在异常激活,可能在胰腺癌的发生发展中起重要作用,有望成为胰腺癌新的治疗靶点

    Abstract:

    Objective:To study the expression of MAP3K10 in human pancreatic carcinoma (PC), and the relationship between MAP3K10 and human PC.
    Methods:The expressions of MAP3K10 protein and mRNA in PC and adjacent non-tumorous tissues were detected by immunohistochemical staining, Western blot and quantitative Real-time PCR.
    Results:(1)Immunohistochemical staining demonstrated that the number of positive staining cells in pancreatic carcinoma (100% strong positive) was notablely higher than that in matched adjacent non-tumorous tissues (only 25% weak posive) (P<0.05);(2)Western blot proved that the expression of MAP3K10 protein in 91.7% of PC, which was higher than that in matched adjacent non-tumorous tissues; (3)qRT-PCR showed that the expression of MAP3K10 mRNA in PC was significantly higher than that in matched adjacent non-tumorous tissues(P<0.01).
    Conclusions:MAP3K10 is abnormally activated in pancreatic carcinoma, might play an important role in the carcinogenesis and progress of pancreatic carcinoma, and is expected to become a new therapeutic target for pancreatic carcinoma.

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林凡| 王敏| 洪晓泉| 李旭| 王欣| 秦仁义. MAP3K10在胰腺癌中的表达及其意义[J].中国普通外科杂志,2011,20(3):257-260.

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  • 收稿日期:2011-01-11
  • 最后修改日期:2011-02-28
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  • 在线发布日期: 2011-03-15
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