Objective:To study the effect of apoptosis in the pathogenesis of great saphenous varicose(GSV) resulting from primary deep venous insufficiency (PDVI). Methods :Apoptosis and Bcl2 expression in the segment of first valve sinus of the GSV of PDVI of lower limbs were detected by transmission electron microscopy(TEM), agarose gel electrophoresis, TUNEL and immunohistochemistry. Results:There were 38 cases of PDVLs in experment group and 5 normal GSV in control group. In experiment group,the apoptosis cells(AC) (6.30±2.70)and apoptosis rate (AR)(0.42±2.12) in the first valve sinus of GSV were significantly higher than those in control group(1.60±0.81,0.21±1.10,respectively)(all P<0.05).In patients with I°,II° blood reflux of deep venous,the AC(3.06±1.65)and AR(0.36±2.41) were significantly lower than those in patients with III°,IV° blood reflux(9.85±3.26,0.48±2.96,respectively)(all P<0.05).
The Bcl2 expressions in the segment of first valve sinus of GSV in patient with PDVI (0.29±1.80) was significantly lower than that in control group (0.35±1.03)(P<0.05). Conclusions:Apoptosis and suppression of Bcl2 expression have important roles in the pathogenesis of PDVI of lower extremities.