Objective:
To investigate the effects and mechanism of magnetic microsphere combination of cisplatin with nano or micron ferric oxide particle on human hepatoma cell(HHC). Methods : A human hepatoma cell line BEL7402 was used in this study, 0.04μg/ml～8μg/ml of cisplatin(CDDP), cisplatin nanoferric oxide magnetic microsphere(nCDDPmm) and microferric oxide magnetic microsphere(mCDDPmm) were administered to the culture solution for culturing the BEL7402 cell as the experimental groups,and in the control groups were administered with cisplatin 0μg/ml and magnetic microsphere without cisplatin respectively. The optic density of viable cell, cytotoxity index, growth curve of cell, cell cycle, proliferation index and apoptosis were assayed by MTT method, cell count and flow cytometry respectively. Results : (1)The viable cell's optic density decreased and the cytotoxity index increased in human hepatoma cell following increasing dosages of CDDP, nCDDPmm and mCDDPmm in culture solution,presenting a dosedependent manner(r value of three drugs were 0.95, 0.91 and 0.89 respective, P＜0.01）. (2)The growth curve of human hepatoma cell numbers developed smoothly, when the dosage of the drugs and timing increased. The cell numbers in nCDDPmm group and mCDDPmm group were higher than that in CDDP group, when dose ≤0.8μg/ml, and timing from 6h to 24h. The cell numbers was no significant difference（P＞0.05）between nCDDPmm and mCDDPmm. (3)Apoptosis rate of hepatoma cell increased (P＜0.01), when dose of CDDP, nCDDPmm and mCDDPmm increased. The apoptosis rate in nCDDPmm group was highter than that in mCDDPmm group (P＜0.01). (4)The G0/G1 phase increased, and S phase decreased, when human hepatoma cell was treated by CDDP,nCDDPmm and mCDDPmm. Conclusions:The antineoplastic effects of nCDDPmm and mCDDPmm depend on the concentration of CDDP, the mechanism is mainly the apoptosis occurred in human hepatoma cell. Ferric oxide particles of nano and micron have faint stimulative proliferation of human hepatoma cell, however，it is enough to eliminate the influence of ferric oxide particle on hepatoma cell as the CDDP released slowly from nCDDPmm and mCDDPmm with time.