Objective:
To study the relationship of expression of angiopoietin gene and angiogenesis in the insitu implanted hepatoma in rats. Methods: Forty Wistar rats were used to establish the insitu implanted hepatoma model by implanting Walker256 hepatoma cell line. The number of microvessel density(MVD) in hepatoma was calculated. The expression of angiopoietin mRNA was observed by insitu hybridization method. Results:The MDV in the implanted hepatoma in the first day of 1, 2 and 3 week postimplantatively were （15±4.3）/Hp，（17±3.6）/Hp，（45±7.8）/Hp respectively. The amount of MDV and expression of Ang1 mRNA were increased significantly and correlated with MDV in the hepatoma tissues and nonhepatoma tissues,and no significant difference between the two types of tissues. Expression of Ang2 mRNA was not seen in nonhepatoma tissues,but in hepatoma tissues,the expression of Ang2 was obvious. Conclusions:Angiopoietin2 mRNA may play a role in the angiogenesis of the insitu implanted hepatoma.