Abstract:Objective:To explore an ideal method of establishing a rat acute hepatic failure (AHF) model. Methods:36 Sprague-Dawley rats were randomly divided into three groups. In modified operation induction group, rat AHF model was established by modified 95% hepatectomy. In the operation, 10ml 5% glucose saline solution per kilogram of body weight was injected into the median lobe hepatic vein of the rat. In traditional operation induction group, 95% liver was resected, but no glucose saline solution was injected. In drug induction group, AHF model was established by injecting 1.2g D-gal per kilogram of body weight intraperitoneally. Operative mortality and 24h survival rate after establishment of models were observed. ALT, total bilirubin (TB), NH3 and blood sugar (BG) were monitored. Results:The operation mortality in traditional operation induction group was higher than that in modified operation induction group (33.3% vs 0%). Survival rate was 0%, 0%and 25% in modified operation induction group, traditional operation induction group and D-gal group 24h later, respectively. ALT and NH3 in the modified operation induction group were significantly higher than those of D-gal group (P<0.05). TBIL and BG levels in the modified operation group were lower than those in the D-gal group, but the difference was not significant (P>0.05). Conclusions:The AHF model established by modified 95% hepatectomy is an ideal one. Injection of 5% glucose saline solution through median lobe hepatic vein at operation can reduce operative mortality.