Abstract:Objective:To investigate the effect of HSP70 gene transfection to peripheral blood cytokines in the early phase of sepsis in rats. Methods:A rat model of sepsis was established by cecal ligation and puncture(CLP),and adenovirus-mediated HSP70 gene transfection was performed in the CLP rats. Serum TNF-α，IL-1β，IL-6，IL-10 were measured before and after HSP70 gene transfection. Results:In CLP rats, serum TNF-α increased at 3h and peaked at 6h（t TNF-α=16.506, P<0.01）. Serum IL-1β began to elevate at 6h and reached the peak at 24h(t IL-1β=22.536, P<0.01). Serum IL-6 was significantly higher than control group at 3h, and peaked at 12h(t IL-6=33.977, P<0.01). IL-10 began to increase at 24h, reached its peak at 48h(t IL-10=15.056;P<0.01), and returned to the level of control group at 72h. In HSP70 gene transfection rats, the pro-inflammation cytokine level of TNF-α at 6h,IL-6 at 12h,IL-1β at 24h was inhibited,but anti-inflammation cytokine level of IL-10 at 48h was not inhibited. Conclusions:HSP70 gene transfection might down-regulate the overexpression of pro-inflammatory cytokines in the body, and the expression of anti-inflammation cytokines was not inhibited. These results suggested that tranfection of HSP70 gene can provide protective effects against sepsis. HSP70 gene transfection might be of clinical relevance in the prevention and treatment of sepsis and MODS.