Objective：To investigate the establishment of a rat model of orthotopic liver transplantation with more complete and easier performance of graft perfusion and easier control of warm ischemic time. Methods：A modification of Kamada twocuff method was used. Cold perfusion of donor livers was done through the ascending aorta. Suture anastomosis of suprahepatic vena cava was performed. Infrahepatic vena cava and portal vein anastomoses were done by the cuff technique. The bile duct was anastomosed with internal stent. The nonheartbeating donor(NHBD) graft model of orthotopic liver transplantation in rats was thus established.Results：The survival rate of recipients grafted from NHBD with perfusion of ascending aorta was 95% (38/40) for 1 day and 85.5% (35/40) for 7 days. Conclusions：The livers of NHBD as grafts harvested with perfusion through ascending aorta had more complete and homogeneous perfusion, perfusion was easier to perform and warm ischemia time was more accurately controlled. The oneweek posttransplantion survival rate was longer than that reported in the literature.