Abstract:Objective:To study the significance of expression of prostate-specific antigen mRNA（PSAmRNA）and estrogen receptor beta mRNA(ERβmRNA) in breast cancer(BC), and the relationship of PSAmRNA expression with subtype estrogen receptor expression. Methods:Reverse transcriptase polymerase chain reaction was conducted to detect the expression of PSAmRNA and ERβmRNA in 35 breast cancer samples,12 juxta-carcinoma breast tissues and 10 breast fibroadenoma samples. Immunohistochemical method was conducted to analyze the expression of ERα and PR protein in 35 BC samples. Results:The level of PSAmRNA in BC tissues was significantly lower than that in juxta-carcinoma breast tissues and breast flbroadenoma tissues（all P=0.00）. The expression of ERβmRNA in BC tissues was significantly lower than that in juxta-carcinoma breast tissues and breast flbroadenoma tissues（all P=0.00）. The expression of ERmRNA in PSAmRNA positive expression BC tissues was significantly lower than that in negative expression BC tissues（P=0.038）. The expression of PSAmRNA in ERα and PR positive BC tissues was higher than that in ERβ and PR negative BC tissues（P=0.001, 0.004 respectively）. Conclusions:PSAmRNA and ERβmRNA in BC tissues is downregulated, and PSA gene can be an important marker that reflects the hormonal status of the BC.