Abstract:Objective:To explore the expression and relationship of PTEN and mTOR in the development of cholangiocarcinoma, determine the effect of the PI3K/PTEN/AKT/mTOR signal transduction on the development of cholangiocarcinoma. Methods:The expression of mTOR and PTEN in the cholangiocarcinoma was detected by the immunohistochemistry and RT-PCR method. Results:Compared to normal tissues, the expression of mTOR gene in cholangiocarcinoma significantly increased, but the expression of PTEN gene decreased. There was a negative correlation between mTOR gene and PTEN gene expression in cholangiocarcinoma. Conclusions:The expression of mTOR gene in cholangiocarcinoma was increased and the expression of PTEN was decreased. It suggested that the mTOR gene and PTEN gene could play an important role in the process of development of cholangiocarcinoma.