Abstract:Objective:To investigate the effect of cyclooxygenase-2 inhibitor Celebrex on the growth of gallbladder carcinoma GBC-SD cell line. Methods:Cell growth suppression was counted by MTT method. Apoptotic index(AI) was evaluated by TUNEL staining. The apoptotic rate was counted by flow cytometry(FCM), fluorescence microscopy(FM) and transmission electron microscopy(TEM). Results:Celebrex inhibiting the growth of GBC-SD cell line was dose-depend. The growth inhibition rate with 40μmol/L,80μmol/L,120μmol/L and 160μmol/L was 18.77%,25.32%,46.58% and 52.19% respectively,（P<0.01）.Apoptotic rate of GBC-SD cell with 40μmol/L,80μmol/L,120μmol/L and 160μmol/L Celebrex was (8.51±1.44)%,(12.40±0.87)%,(26.37±1.72)% and (43.21±0.39)% respectively, and was significant difference in relation to the control group (4.87±0.55)%. Apoptotic bodies and cell shrinkage and fragmentation was observed by FM and TEM. Conclusions:Celebrex can effectively inhibit the growth of gallbladder carcinoma GBC-SD cell line, and induce the apoptosis.