Abstract:Objective:To evaluate the effect of radiofrequency ablation (RFA) comblined with high frequency hyperthermia (HFH) and transcatheter arterial chemoembolization (TACE) on rabbit liver VX2 tumor.
Methods :Rabbit liver VX2 tumor models were divied into the following group: Group A, RFA+TACE+HFH; Group B, RFA+HFH; Group C, FA+TACE; Group D, TACE+HFH.The changes of ALT, hot shock protein 70(HSP 70)expression of liver and dendritic cells (DCs) expression of spleen before and after therapy were observed.
Results:(1)ALT: value of ALT was markedly elevated on 1d after therapy in all groups, but was greatest in Group A and also was the slowest to recover at 14 d; the degree of elevation was least in Group B and was the fastest to recover (P<0.05). (2)HSP70 expression: It was markedly elevated in Group A and was still the highest at 14 d; the degree of elevation was least in Group D and was the lowest at 14 d (P<0.05).(3).DCs expression: There was no difference between Groups A and B at 14 d and 7 d (P>0.05); and Groups C and D were markedly lower than the A and B group, and declined more quickly (P<0.05).
Conclusions:RFA+TACE+HFH can effectively activate the expression of Hsp70 and DCs, and enhance antitumorimmunopotency of the organism, but, at the same time, can most markedly damage liver function. Therefore, their combined use should be given an all-round consideration.