Abstract:Objective:To explore the expression of B7-1 and B7-2 in cold ischemia/reperfusion (I/R) injury in the rat liver and its immunological role.
Methods :Thirty rats were divided into three groups randomly. Group A: sham operation group; group B: cold ischemia for 20 minutes and reperfusion; and group C: cold ischemia for 30 minutes and reperfusion. The expression of B7-1 and B7-2 mRNA in the livers was analyzed by real-time reverse transcription polymerase chain reaction(RT-PCR).
Results:B7-1 mRNA expressions in group B and C were 0.529±0.089 and 0.618±0.074 respectively, which were significantly higher than those in group A(0.131±0.012, P<0.01). B7-2 mRNA expressions in group B and C were 0.474±0.132 and 0.682±0.095 respectively, which were significantly higher than those in group A(0.163±0.054, P<0.01). In addition, both B7-1 and B7-2 levels were higher in group C than group B.
Conclusions:Cold ischemia/reperfusion injury may increase the immunogenicity of the liver by upregulating B7-1 and B7-2.