Abstract:Objective:To investigate the protection effect of β-aescin on rat intestine ischemia /reperfusion (I/R) injury.
Methods :Eighty-eight male wistar rats were randomly divided into two groups, then they were distinguished by different time point after occurrence of reperfusion at 0 h, 1 h, and 2 h. We observed the changes of the serum tumor necrosis factor-α， interleukine-6, the apoptosis of intestine mucosal epithelial cell, intestinal microvascular permeability, and the survival rate.
Results:The group with β-aescin pretreatment was observed to have reduction of intestinal histopathological damage, and significant decrease of Haglund scores, intestinal microvascular permeability, intestinal water content and the level of TNF-α and IL-6 (P＜0.01), and inhibition of intestinal mucosa cell apoptosis (P＜0.05) and mortality rate during 36 hours (P＜0.01) compared to controlled group with I/R after reperfusion.
Conclusions:The β-aescin pretreatment could protect the intestinal mucosa from I/R injury, and it might be related to suppression of proinflammatory factors, reduction of intestinal mucosa cell apoptosis and decreased intestinal microvascular permeability.