Objective:To search the effect of venous thrombosis (VT) on the endothelial cells (ECs) of nonthrombosis vein segment (ECNT).
:Methods :Ninty rats were divided into a normal group, a thrombosis group established by ligating a section of iliac vein, and a sham operation control group. An electroscope was used to detect the ECs of target vessels morphological changes. The Ecs were collected from nonthrombotic superior vena cava and iliac veins by enzymolysis and studied by flow cytometer to detect the positive rate of apoptosis.
:Results:The morphology of endothelial cells in the nonthrombus vein segments of the thrombosis group showed marked changes compared to those of sham operation control group. Comparison of thrombosis group and sham aperation control group showed significant difference in early apoptosis rate at 1, 2, 4, and 7 d after operation (all P＜0.05, 0.01, P＜0.01).
:Conclusions:Local deep vein thrombosis can lead to endothelial cell injury and early apoptosis of distant vein segment.