Abstract:Objective:To explore the therapeutic effects and mechanisms of somatostatin on liver injury in acute hemorrhagic necrotizing pancreatitis(AHNP) in rats.
Methods :Murine model of AHNP was established by retrograde injection of artificial bile into the biliopancreatic duct.The animals were divided into sham operation group(S group), AHNP saline injection group(AHNP group), and AHNP octreotide treated group. TLR2,4 mRNA expressions and NF-κB expressions in the liver tissue at 3,6 and 12h after operation were measured.
Results:TLR2,4 mRNA in liver tissue in the AHNP group began to rise at 3h，and peaked at 12 h（P<0.01）;NF-κB expression in liver tissue also began to rise at 3h, and peaked at 6h. In contrast, in the groups treated with Somatostatin,TLR2,4 mRNA and NF-κB expression in the liver tissue decreased at each time point (P<0.05).
Conclusions:Somatostatin has a protective effect on AHNP. The mechanism may be by inhibition of the secretion and activation of elastase, inhibition of Toll receptor and NF-κB expression and decrease of inflammatory reaction, and decrease the liver injury.