Abstract:Objective:To investigate the expression of Pirh2mRNA in breast cancer tissue and its correlation to genesis and development of breast cancer.
Methods :A retrospectively analysis of pirh 2 mRNA expression in 87 cases of stage I and II breast cancer, 27 cases of stage III and IV breast cancer was made, and 10 cases of fibroma tissue and 6 cases of normal lacteal gland tissue as control, all of which were from Department of Breast Surgery, XiangYa Hospital in recent 10 years. The in situ hybridization was used to detect the expression of Pirh2mRNA.
Results: The positive rate of Pirh2mRNA in breast cancer tissue was 43.9 %(50/114), 1 positive in 10 cases of fibroid tumor, and no expression in normal lacteal gland tissue. The expression of Pirh2mRNA in breast cancer was significant higher than that in fibroid tumor and normal lacteal gland (P=0.004). The positive rate of the stage I or II breast cancer group and was 37.9% (33/87) and of stage III or IV was 63% (17/27) respectively, with a significant difference (P=0.022). In the 60 cases with metastasis occurred in less than 5 years the positive rate of Pirh2mRNA was 63.3% (38/60), and the positive rate of the 54 non-tumor survivors was 22.2% (12/54)（P=0.000）.
Conclusions:Pirh2mRNA is more highly expressed in breast cancer, while there is almost zero expression in lacteal gland fibroid and normal tissue. The positive expression in stage III or IV is significantly higher than breast cancer of stage I or II. Pirh2mRNA is probably closely correlated to the genesis and development of breast cancer.