Abstract:Objective:To investigate the expression of BRMS1 in different metastatic breast cancer cells and its relation to HDAC activity.
Methods :The gene expression of BRMS1 in 3 types breast cancer cell lines〔non-metastasis MCF-7（A cell line）, low metastasis MDA-MB-453（B cell line）and high metastasis MDA-MB-231（C cell line）〕 was determied by RT-PCR technique; the protein expression of BRMS1 was measured with Western blot technique and HDAC activity by enzyme linked immunosorbent assay.
Results:（1） Gene expressive ratio of BRMS1 was 3.1∶2.0∶1.0 in the A、B、C cell lines, respectively. The gene expression of BRMS1 in the C cell line decreased 210% compared to A cell line, and expression of BRMS1 was markedly reduced as the degree of metastasis increased (P＜0.001). （2）protein expression ratio of BRMS1 was 3.2∶1.9∶1.0 in the A、B、C cell lines. The protein expression of BRMS1 in the C cell line decreased by 220% compared to A cell line, and protein expressive of BRMS1 was reduced in relation to metastasis degree of breast cancer cell line (P＜0.001). （3）HDAC activity ratio was 1.0∶1. 8∶2.6 in the A、B、C cell lines . HDAC activity in the C cell line increased 160% compared to A cell line,HDAC activity increased in relation to metastasis degree of the cell line (P＜0.001).
Conclusions:（1）Gene and protein expression of BRMS1 is reduced according to the metastasis degree increasing of the cell line. （2）HDAC activity increases with increase of degree of metastasis of the 3 cell types，and suggests that BRMS1 may regulate breast cancer metastasis via HDAC activity.