Abstract:Objective:To investigate the role of AGE-RAGE system on intimal hyperplasia in autogenous vein graft of diabetic rats.
Methods :The experiments were performed on 40 male Sprague-Dawley rats which divided into two groups: diabetes group and control group. Autogenous vein graft model was established in all rats. Specimens were harvested 7 days and 14 days after surgery for histological examination, and RAGE, NF-κB p65 and VCAM-1mRNA were measured by semi-quantitative reverse transcription-PCR. Western Blotting and immunohistochemistry were used to detect the protein expression of RAGE, NF-κB p65 and VCAM-1. At the same time, the levels of serum AGE were tested.
Results:Compared with non-diabetic rats of control group, the intimal hyperplasia of vein graft in diabetic rats obviously increased (P<0.05). At the same time, themRNA and protein expression of RAGE, NF-κB p65 and VCAM-1 obviously increased (P<0.05), and the level of the serum AGE obviously increased (P<0.05).
Conclusions:AGE-RAGE system activated NF-κB and enhanced the expression of VCAM-1, which might be the cause of increased intimal hyperplasia in autogenous vein graft of diabetic rats.