Abstract:Objective:To study the effects of GLP-2 on modulation of intestinal mucosa epithelium tight junction in obstructive jaundice rats.
Methods :The obstructive jaundice model of rats was set up. At 10 days after operation, the rats were randomly divided into 4 groups: normal group, jaundice group,experiment group A and B. Each group consisted of 10 animals.The animals of jaundice group had subcutaneous injection of 0.5ml 0.01mmol/LPBS,experiment group A had intraperitoneal injecton of 0.5 mL 250 g/(kg.d) GLP-2; experiment group B intraperitoneal injectied 0.5ml 125g/ (kg.d)GLP-2, b.i.d×7. Immunohistochemistry and Western-blot were used to examine the distribution and expression of tight junction proteins ZO-1, occludin and Claudin-1,-4 in the terminal ileum mucosa. Image analytical system and statistics software were used to analyze the results quantitately.
Results:In normal group, the strong positive expression ratios of ZO-1,occludin and claudin-1 were 70.0%,80.0% and 70.0% respectively. In jaundice group, ZO-1 and Occludin staining appeared discontinuous and vague. ZO-1, occludin and claudin-1 staining recovered somewhat after exogenously supplied GLP-2 in experiment group A. The strong positive expression ratios were obviously higher in experiment group A than those in the jaundice group (20.0% vs 80.0%，30.0% vs90.0%,20.0% vs 80.0%,all P＜0.05). The distribution and expression of Claudin-4 altered insignificantly. There were no noticeable changes in the expression and quantity of tight junction proteins in experiment group B. The same outcomes were obtained by quantitative analysing Western blot images.
Conclusions:Exogenous supplemented GLP-2 in obstructive jaundice can influence the expression and distribution of the tight junction proteins ZO-1, occludin,Claudin-1 of intestinal mucosa. This suggests that GLP-2 could recover and maintain the integrity of intestinal mucosa epithelium barrier.