Abstract:Objective:To explore the role of apoptosis and the expression of X-linked inhibitor of apoptosis protein (XIAP) in polymorphonuclear neutrophils (PMNs) during acute pancreatitis (AP).
Methods :Blood from normal control (NC, n=15), mild acute pancreatitis (MAP, n=15) and severe acute pancreatitis (SAP, n=15) were collected. PMNs apoptosis was detected by flow cytometry. PMNs were isolated from each group and XIAP mRNA and protein levels were assessed by RT-PCR and Western Blotting.
Results:PMNs apoptosis in SAP group was (2.15±0.40)%, MAP group was (4.16±0.14)%，NC group was (4.31±0.12)%. PMNs apoptosis rate in SAP and MAP groups was decreased compared to NC group (P<0.05), in SAP group was markedly decreased compared to MAP group (P<0.01). The expression of XIAP in SAP and MAP groups was increased as compared to NC group (P<0.01), and XIAP expressed in SAP was markedly increased compared to MAP group (P<0.01).
Conclusions:XIAP may contribute to the severity of acute pancreatitis through suppression of apoptosis of PMNs.