Abstract:Objective:To study the relationship between chemoresistance and the expression of survivin，induced by gemcitabine, in pancreatic carcinoma cell line PANC-1.
Methods :The pancreatic carcinoma cell line PANC-1 was cultured by RPMI 1640 and gemcitabine with the concentration of 1 μg /ml and 10 μg /mL respectively, and survivin expression was examined by RT-PCR and Western blot. The relationship of the survivin level of the cell and its chemoresistance to gemcitabine was analyzed.
Results:Tweenty-four and 48 hours after culture with gemcitabine concentration of 1 μg /ml and 10 μg /mL respectively, survivin mRNA level was increased (1.34±0.12),(2.40±0.17),and(3.33±0.20),( 4.41±0.18) folds, and the protein expression was enhanced (1.20±0.07),(1.48±0.19),and(2.90±0.04),(4.50±0.20) folds respectively. There was a significant difference between the two groups (P＜0.05).
Conclusions:The intensity of pancreatic carcinoma cell lines chemoresistance could be enhanced by the action of gemcitabine on the up-regulated expression of survivin.