Abstract:Objective:To explore the effects of different preparation in adherent cells on the immunohistochemical display of membrane protein under light microscope.
Methods :Anchorage-dependant cells were cultured which included three groups: Gastric epithelial cell GES-1, gastric cancer cell SGC7901 and gastric cancer cell SGC7901/VCR. The cells were seeded on coverslips or cytospinned on the microscopic slides, and the MDR-1 detected by immunohistochemical method. Some of the specimens were re-stain with methyl green for afterstain. Finally, semiquantitative analysis was carried out.
Results:The MDR-1 immunoreactivity(IR) of specimens with re-stain was higher than that without re-stain in the same cell line and the same preparation, no matter whether the cells were seeded on coverslips or cytospinned on the microscopic slides. As to the same cell line, no matter whether afterstain or not, the MDR-1 IR in the cells cytospinned on the microscopic slides was higher than that in the cells seeded on coverslips. The MDR-1 expression level was different among three group cells, but their tendency of change was consistent in the same preparation and the same re-stain.
Conclusions:The adherent cells cytospinned on the microscopic slides have much better immunohistochemical display of membrane protein than those seeded on coverslips under light microscope.