Abstract:Objective:To explore the feasibility of constructing PTLD model.
Methods :The peripheral blood lymphocytes of 40 EBV(+) volunteers were injected intraperitoneally into 3 SCID mice respectively. IgG determination was utilized to confirm the reestablishment of human immunocell in SCID mice. The tumor tissue of the dying or dead mice were retrieved,and then the establishment of LPD model was determined by pathological section and immunohistochemistry.
Results:The IgG level in the blood serum of SCID mice was (750.0±13.2)μg/mL and (1 050.0±14.6)μg/mL respectively at 8 weeks and 12 weeks post transplantation. Pathological section of retrieved tumor tissue indicated that the tissue was large-cell lymphoma with high mitotic rates.The tumors were CD20 positive and LMP-1 positive. Among the 40 volunteers, human peripheral blood lymphocyte from 10 volunteers (25%) produced LPD rapidly(median time to LPD, 8 weeks)and with high penetrance (median, 100%); whereas 20% (8 of 40 volunteers) developed LPD tumor later (median 12 weeks),and only in partial mice (median penetrance, 55%); HuPBLs from the remainder of the donors(22 of 40,55%) produced no LPD after 16 weeks.
Conclusions:PTLD model can be successfully established by intraperitoneal injection of EBV(+)huPBL in SCID mice.