Abstract:Objective:To comparatively investigate the protective effect of HGF and SAM on liver regeneration and liver function in cirrhotic rats after hepatectomy.
Methods :Liver cirrhosis of the rat was successfully induced by injection of CCL4.Then 30% partial hepatectomy was performed in all rats.Cirrhotic rats were divided into 4 groups, namely, cirrhotic group(normal saline 5 mL/d IM, n=16), HGF group(HGF 0.65 mg/(100 g·d) peritoneal injection, n=16), SAM group（SAM 20 mg/(kg·d) IM, n=16）and HGF+SAM group(HGF 0.65 mg/(100g·d), SAM 20 mg/(kg·d), n=16).Animals were sacrificed on the 15th post-operative day to take samples for detection of liver function(AST, ALT, ALB and TB).Liver tissues were also observed under light microscope and electron microscope.
Results:In HGF group, SAM group and HGF+SAM group, valus of AST, ALT and TB were decreased significantly as with cirrhotic group(P＜0.05), and concentrations of ALB were increased significantly(P＜0.05).
Conclusions:The study suggests that the liver function and regeneration of cirrhotic rats have impaired after partial hepatectomy. However HGF and SAM have the efficacy of enhancing liver regeneration and improving liver function.