Objective:To investigate the cytotoxic and chemo-sensitivity enhamcement effect of cucurmosin（CUS） on pancreatic cancer cell line PANC-1 in vitro.Methods:The cytotoxic effect of the different concentrations of CUS on PANC-1 cells in different time periods (24, 48 and 72 hours) was detected by means of MTT assay，and the chemo-sensitivity enhancement effect of CUS on gemcitabine(GEM) was evaluated by combination-index method of Chou and Talalay.Results:Cucurmosin significantly inhibited the proliferation of pancreatic cancer cell line PANC-1 in dosage-and time-dependent manner. After exposure to 0.3125,0.625,1.25,2.5,5.0,10.0,20.0,40.0 and 80 μg/mL of CUS, the inhibitory rate of the cell growth at 24 hours was 9.16％,11.9％,14.4％,20.2％,32.0％,36.4％,44.1％,50.3％and 58.6％respectively. The 50% inhibitory concentration (IC 50) at 24、48 and 72 h was 40.24 μg/mL,8.24 μg/mL and 1.17 μg/mL respectively. The combination of CUS and GEM at different concentrations showed synergic effect on inhibiting PNCA-1 cell growth (CI<1).The 50% inhibitory concentration (IC 50) of GEM decreased from 36.76 nmol/L to 12.14 nmol/L(less than 67.0%) when combined with CUS.Conclusions:Cucurmosin can significantly inhibit the proliferation of PANC-1 cells in dosage-and time-dependent manner in vitro,and combination with gemcitabine increases its cytotoxic action and provides chemo-sensitivity enhancement effect on pancreatic cancer cell line PANC-1.