Objective:To study the effects of hSSTR2 gene transfection for downstream proteins in pancreatic cancer cells Panc-1 in vitro and its clinical significance.Methods:The full length hSSTR2 cDNA was introduced into pancreatic cancer cell line Panc-1 by adenovirus mediated transfection.Comprehensive analyses of proteins were focused on total protein spots exhibiting statistical alternations between the experimental group and vector control by fluorescent two-dimensional difference gel electrophoresis analysis.Proteins were identified by peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Western blotting was performed to verify the differential expression of vimentin.Immunohistochemistry was performed to detect the expression of vimentin in pancreatic carcinoma tissue, and its clinical significance was analyzed.Results:There were 21 statistically significant different protein spots.Of them, 18 different points were selected(greater then 1.3-fold); of the 18 protein spots,13 proteins including the regulatory proteins of translation, metabolic enzymes, the associated proteins about cell communication and signal transduction, the cell structural proteins, molecular chaperones, the proteins with the GTPase activity, and power protein were identified by mass spectrometry.Western blot results revealed that the downregulation of vimentin in pancreatic cancer cells with transfected hSSTR2 gene.Immunohistochemistry results revealed that vimentin was highly expressed in poorly differentiated pancreatic cancer cells.Conclusions:These differential expressed proteins, such as vimentin, may serve as new sensitive therapeutic targets in pancreatic cancer.Vimentin may also serve as a biomarker for predicting the degree of malignancy of pancreatic cancer.