Objective:To observe the influence of CO2pnemumoperitoneum models on the growth and metastasis of seeded SGC-7901 cells in nude mice.
Methods:The SGC-7901 cells suspension was injected into the peritoneal cavity of 27 nude mice(2×106 cells/mouse), then the mice were randomly divided into three groups (9 cases per group).Control (A) group,the mice was laparotomy only;and CO2 pnemumoperitoneum was established with pressure 5 mmHg (group B) and 8 mmHg (group C), the treatment time was 1 h. The control group underwent a midline incision，which was closed after 1 h. Four weeks later, all of the nude mice were killed，and the number of macroscopic abdominal cavity nodules，the total body weight, the mean max diameters and distributing ranges of tumor mass were observed and measured.The rate of viscera (liver, spleen, peritoneum and mesentery) metastasis was also recorded in each group, and the HpamRNA expression in tumor tissue was measured by RT-PCR。
Results:The number of abdominal cavity nodules in each group was not significantly different (control group: 7.4±2.5, 5 mmHg group: 7.3±2.4, 8 mmHg group: 7.5±2.1. P＞0.05). also the total body weight, the mean max diameters and distributing ranges of metastasis showed no difference between each group（P＞0.05）. The HpamRNA expression in gastric cancer tissues showed no significant difference in each subgroup(P＞0.05).
Conclusions:CO2 pnemumoperitoneum do not accelerate the SGC-7901 cells growth invasion and metastasis in vivo，also it had no significant effect on HpamRNA expression.