Objective:To construct SiRNA recombinant expression vector targeting survivin gene and study its effect on inhibition of proliferation of human colon cancer cells and enhancement of chemotherapy sensitivity.
Methods:SiRNA recombinant expression vector targeting survivin gene was constructed and transfected into human colon cancer cells.The effect of SiRNA recombinant expression vector was detected by RT-PCR and western blot. Enhancement of chemotherapy sensitivity was observed by MTT reduction assay and flow cytometry.
Results:SiRNA recombinant expression vector targeting survivin gene was constructed successfully confirmed by restriction endonuclease and sequence analysis, and its inhibition ratio on survivin mRNA and protein levels was 36.33％ and 44.65％, respectively. It could enhance the inhibitory effect of 5-Fu on growth of colon cancer cells.
Conclusions:The SiRNA recombinant expression vector targeting survivin gene can effectively inhibit the expression of survivin gene. It also can synergetically enhance the chemotherapy sensitivity and apoptosis induction of 5-Fu on human colon cancer cells.