Objective:To observe the changes of LoVo cell growth by galectin-1.
Methods:Eukaryotic expression vector of galectin-1 was constructed and transfected into LoVo cells using lipofectamineRM 2000. Immunochemistry was employed to detect galectin-1 expresson. LoVo cell proliferation and apoptosis were observed.
Results:Galectin-1 eukaryotic expression vector pEGFP-C1/GAL1 was successfully constructed. Three cell clones, p-GAL1-LoVo and p-LoVo,transfected with pEGFP-C1/GAL1 and pEGFP-C1 correspondingly,and LoVo were cultured successfully. Galectin-1 expression downregulated Bcl-2 level only occurring in p-GAL1-LoVo cells. p-GAL1-LoVo cell proliferation was similar to p-LoVo and LoVo cells,but p-GAL1-LoVo cell apoptosis was increased (9.61±0.56)%,as compared with p-LoVo and LoVo cells,［(3.56±0.53)% and (3.46±0.46)% respectively］(P<0.001).
Conclusions:Galectin-1 induces cell apoptosis possibly by downregulating Bcl-2 expresson.