Objective:To explore the expression and effects of RSp1 and β-catenin in the intestinal epithelium with intestinal ischemia-reperfusion injury (IIRI) in of mouse.
Methods:Fifty healthy male kunming mice were randomly divided into control group（n=10）and experimental group (n=40). All mice in control group were only subjected to laparotomy, while the other mice underwent 20 minutes of intestinal mesenteric artery occlusion followed by 6 hours (group A),12 hours(group B), 24 hours(group C) and 48 hours(group D) of reperfusion. RT-PCR was used to detect RSpo1 and β-catenin in small intestine in intestinal ischemia-reperfusion groups and in control group.
Results:The villous heights of intestinal in experimental groups were significantly lower than that in control group（P<0.05~0.01）.The varying trend of β-Catenin expression in experimental groups was the same as villous height,its expression was significantly weaker than that in control group（P<0.01），and increased gradually along with the reperfusion time. Compared with control group, RSpo1 expression decreased rapidly at 6h(P<0.05),then increased gradually at 12 h, reached the peak level at 24h(P<0.01)followed by rapid decrease and remained significantly lower than that in control group at 48h(P<0.05).
Conclusions:Intestinal ischemia-reperfusion injury may inhibit β-catenin and RSpo1 expression in early stage, but it increase in middle stage. Both of them may be related with the proliferation and differentiation of intestinal stem cells and role an important part in the process of repair after mucosal damage.