Objective:To investigate the regulative effect of the mfn2 gene on RECK expression and the activity of MMP-9 and MMP-2 in breast carcinoma cell (MCF-7 cell line).
Methods :The pEGFP-mfn2 was transfected to MCF-7 cells with lipofectamine2000, the mRNA expression of mfn2 and RECK was assessed with RT-PCR, Western analysis was used to detect the protein expression of the mfn2 and RECK gene;and MMP-9 and MMP-2 activity was analyzed using gelatin zymograph.
Results:After transfection of pEGFP-mfn2, the stable expression of Mitofusin-2 gene was detected in MCF-7 cells, the expression of RECK mRNA level and protein were increased significantly, while the MMP-9 and and MMP-2 activity level was markedly lower in the transfected Mitofusin-2 gene group than in the control(P<0.05).
Conclusions:The mfn2 gene induces the expression of the RECK gene and inhibits the activity of MMP-9 and MMP-2 in MCF-7 cells, which might be a new mechanism of its anti-tumor effect.