Objective:To study the expression level and effect of hNIS gene to induced125I uptake after transfecting hNIS gene into cholangiocarcinoma cell line QBC939.
Methods :The hNIS cDNA sequences by amplification were ligated with plasmid pMD18-T, selected, subcloned and ligated with eucaryotic expressing plasmid PDC316, using lipofectamine to transfect eucaryotic expressing plasmid vector hNIS-DNA-PDC316（experimental group）and plasmid PDC316（control group）into cholangiocarcinoma cell line QBC939, establishing new cell lines(QBC939-A,QBC939-B) and QBC939-C (control).The expression level of hNIS gene was detected by semi-quantitative RT-PCR in 2-, 3-, and 6-day, and the effect of induced125I uptaking was studied on the 1-,2-, 3-, and 6 day in cell lines of vitro culture.
Results:QBC939-A stably expressed hNIS-DNA-PDC316.The expression level on QBC939-A compared with QBC939-B and QBC939-C had significant difference （P<0.05）, the peak of induced125I uptaking was detected on the 3rd day in QBC939-A, and cell line QBC939-A accumulated 14 and 16 times more radio-iodide in vitro than QBC939-C and QBC939-B respectively.
Conclusions:Tramsfer of hNIS gene into cholangiocarcinoma cells can induce125I uptake in the short-term, This study can be as a foundation for study of targeting action to bile duct carcinoma using radionuclide125I.