Objective:To investigate the effect of dynamic changes of blood fiow and blood pressare, and changes of muscle tissues on rat hindlimb acute ischemia,and provide conditions for study on consequent intervention.
Methods :An acute hindlimb ischemia model was prepared in 10 rats by ligation and excision of left femoral artery and its branches. Doppler scan blood flow and pressure analysis were performed 2 d 7 d, 14 d, 28 d, 42 d, and 49 d after operation. The animals were sacrified post-angiography. H.E stain of muscles was then done.
Results:All the animals survived after operation. Some showed intermittent claudication and cyanosis(n=2), pallor (n=3),and muscle atrophy (n=1). Tissue examination showed there was necrosis and inflammation of skeletal muscle. The blood flow markedly decreased 7days after operation, reached to the lowest point at 4weeks, and recovered almost 90% at 49days after operation. The blood pressure, which reached the lowest at 14days, recovered to normal at 49days.
Conclusions:It is feasible to establish rat hindlimb ischemia model by ligation and excision of femoral artery, but blood flow and pressure can recover after 49days,and the time for intervention is 2-4 weeks after operation,when is the most serious ischmia time.