Objective:To study the effect of combination of recombinant human hepatocyte growth factor (rhG-HGF) and fibroblast growth factor (bFGF) on angiogenesis in limb ischemic mouse model.
Methods :Eighty unilateral ischemic lower extremity mouse models established by operation were randomly divided into four groups:saline group(n＝20), bFGF group (n＝20), rhG-HGF group (n＝20), and rhG-HGF and bFGF group; ischemic/normal limb Doppler blood flowmetry ratio and capillary density were campared, postoperatively.
Results:At 4 weeks after operation, the ischemic/normal limb Doppler blood flowmetry ratio and capillary density Were as follows: rhG-HGF+bFGF group>rhG-HGF group>bFGF group>saline group (all P<0.05).
Conclusions:The stimulatory effect of rhG-HGF on angiogenesis is stronger than that of bFGF,while the combination of rh-HGF and bFGF has a significant synergistic effect and can more markcdly improve the vascular condition in limb ischemia.