Objective:To study the feasibility of cultivaed venous endothelial cells (VEC) transplanting to allogenic vein.
Methods :Endothelial cells were gained from veins after digestion with 0.2％collagenase. After VEC was cultivated,they were seeded into allogenic vein, which was lack of endothelium, with high density, and continuitly cultured in endothelial cell medium. The multiplied cells were lined in vitro onto the luminal surface of allogenic vein that was disposed by freeze-drying and radiation. The cultivated cells were confirmed as endothelial cells by von Willebrand factor.
Results:Primary endothelial cells were obtained after the in vitro cultivation, and their number was successfully expanded to meet the needs of the experiment. The function to secrete von willebrand factor showed no significant change (P>0.05). After allogenic vein transplantation, the cells grew normally. The funtion to excrete von Willebrand factor was same as before.
Conclusions:Endothelial cell can grow well in vitro with no change in secretory function, and have exellent growth on allogenic vein.