Objective:To prepare magnetic adriamycin-verapamil-albumin nanoparticles(MAVANs) and measure the physical property of the MAVANs，such as magnetism，drug loading(DL)and size of nanoparticles in order to direct its further experimental study on animals and its application in clinical practice.
Methods :Commercial cottonseed oil was refined, then mixed with adriamycin,verapamil, human albumin and Fe3O4 magnetic powder according to appropriate proportion, and MAVANs was prepared by using a heat denaturation technique. The size and shape of nanoparticles were determined by electron transmission micro-photography.The magnetic response and structure of the MAVANs were analyzed by upside-down microscope.The standard curve was plotted. The drug loading of verapamil in the MAVANs was quantified by high performance liguid chromatography. The drug loading of adriamycin was quantified by a standard spectrophotonuorometry.
Results:The nanoparticles featured homogenous size, regular and spherical shape, and good magnetic response.The mean diameter of nanoparticles was 0.5μm. The loading amount of verapamil was 0.8%.The drug loading of adriamycin was 1.25%.
Conclusions:The heat denaturation technique is feasible to prepare the nanoparticles which can load two kinds of drug with good physical property.The process is simple and the testing condition is easy to control.