To study the surgical technique of construction of orthopic liver transplantation model in mice by a single operator.
Methods :On the basis of two-cuff technique,running suture was used to reestablish the suprahepatic vena cava(SHVC),"two-cuff"technology to reestablish the portal vein（PV） and infrahepatic vena cava（IHVC）,and"stent"to reestablish the bile duct.Operation was performed in 70 mice, the 24 h,1 week,1M postoperative survival rate were noted, and hepatic function and pathological change were observed.
Results:The 24 h,1 week and 1M survival rate was 95.7%, 90.9%, 85.1%，respectively. The ALT increased gradually in the first postoperative week,and dropped to normal level in the first month. Pathology showed the structure of liver tissue was fine.
Conclusions:The method is an ideal mothod to establish the orthotopic liver transplantation model in mice, because it has high survival rate, good stability and is easily replicated.