Objective: To explore the effect and mechanism of wortmannin  on the human gastric carcinoma cells.
Methods: SGC7901 cells were treated with 10nmol/L,30nmol/L and 60nmol/L of wortmannin,a specific inhibitor of AKT, for different time periods. Cell viability was estimated by MTT assay. Western blot was used to detect the level of NF-κB neucleoprotein and reverse transcriptasepolymerase chain reaction(RT-PCR) was used to determine transcription of NF-κB mRNA.
Results: All three different concentrations of wortmannin could inhibit the growth of SGC7901 cells,and the depression effect obviously depended on time and drug dose(P＜0.01 and P＜0.05).However, the cell viability was no change in the control group(P＞0.05). As the action time of wortmannin was prolonged, neucleoprotein and mRNA expression of NF-κB significantly decreased compared with the control (P＜0.05).
Conclusions: The findings suggest that wortmannin can inhibit the growth of gastric carcinoma SGC7901 cells,and the action may depend on an AKT/NF-κB pathway in gastric carcinoma cells.