Objective: To investigate the expression and significance of fragile histidine triad(FHIT) gene in the cholangiocarcinoma cell line QBC939.
 Methods: The eukaryotic expression vector was transfected into QBC939, real-time RT-PCR was used to detect the change of FHIT expression, and direct counting method under microscope was used to detect the change in the biological behavior of cells.
 Results: After transfection, the expression of FHIT mRNA increased by 2.53-fold, but the cell clones were significantly less ［(11.2±1.3) vs.(10.5±1.1) vs.(6.3±1.0)，P＜0.05］.
 Conclusions: The expression of FHIT gene in QBC939 is attenuated, but after its expression is regained it can inhibit the formation of cell clones.