Objective:To detect the inhibitory effect of HSP27-siRNA on hepatocellular carcinoma (HCC) cells.
Methods:HCC cell QGY lines were cultured with HSP27-siRNA in a differtent range of concentration  for various time periods.Cell activity was studied by MTT.The changes of cell cycle and apoptosis were analyzed by FCM. RT-PCR was used to detect the effect of HSP27-siRNA on QGY cell expression of HSP27 mRNA. Western blot was used to detect the inhibition efficiency of HSP27-siRNA on HSP27protein.
Results:The proliferation of QGY cell was inhibited by HSP27-siRNA,and HSP27-siRNA decreased the expression of HSP27 protein.HSP27-siRNA inhibited the proliferation of QGY cell line and induced apoptosis in vitro, and its effect was both dose-and time-dependent.
Conclusions:HSP27-siRNA can inhibit proliferation and induce apoptosis of HCC cancer cell lines. Thus, it may become a method for effective treatment of HCC cancer.