Objective:To investigate the effect of Bmi1 on proliferation of rat adult hepatic stem cells.
Methods:Bmi1 siRNA was transfected into rat adult hepatic stem cells OC3. Then the expression of Bmi1 in OC3 was detected by real time PCR; the proliferative capability was detected by CCK8; and the cell cycle was detected by flow cytometry 24 h after transfection.
Results:The decreased expression of Bmi1 was associated with decreased viability of OC3 cells ［inhibition ratio: （20.35±0.132）%］ and the number of cells with S phase［（24.48±0.96）%］ was decreased significantly.
Conclusions:Knockdown of Bmi1 can inhibit the proliferative capability of rat hepatic stem cells.