Objective:To investigate the effect of SH2-B on proliferation and cell cycle of colon caner HT-29 cells.
Methods:Screening of the SH2-B low expression colon cancer cell line (HT-29) by immunofluorescence assay was done. pcDNA3.1-SH2-B was transfected into HT-29 cells using LipofecTAMINE 2000. After gene transfection, SH2-B expression was detected by Western-blotting,  cell proliferation was measured by MTT assay, and cell cycle was analyzed by flow cytometer.
Results: Immunofluorescence assay results showed that SH2-B lowly expressed in HT-29 cells.  after being transfected with pcDNA3.1-SH2-B,  SH2-B expression dramatically increased in HT-29 cells, and HT-29 cells proliferation was significantly enhanced (P＜0.05). Cell cycle analysis showed that S stage cells were significantly increased in the SH2-B transfected group (39.4±2.31) % compared with the blank control group (18.6±4.25)% and vector control group (11.1±2.39)% (P＜0.05).
Conclusions:SH2-B may enhance colon cancer HT-29 cell proliferation and promote cell cycle.