Objective: To construct siRNA recombinant expression vector targeting HIF-1α gene.
Methods: Genome sequences of HIF-1α gene were retrieved from gene bank, and four pairs of oligonucleotides were synthesized and inserted into plasmid pGensil-1. Then, DH5α strains were transformed, and plasma was extracted and identified by restriction endonuclease and sequence analysis.
Results: It was confirmed by sequence analysis that siRNA recombinant expression vector targeting HIF-1α gene coincided completely with the designs.
Conclusions: The siRNA recombinant expression vector targeting HIF-1α gene has been constructed successfully and lays a foundation for furher study on the biological behavior of tumors.