Objective:To investigate the mechanism of the cell cycle-associated gene expression regulated by Apr-1 gene in QBC939 cholangiocarcinoma cells. 
Methods:Apr-1 gene was transfected into QBC939 cells by using liposomes to establish a QBC939 cell model (QBC939-Apr-1) stably expressing Apr-1 gene. Then, the regulatory effect of Apr-1 gene on the expression of cell cycle-associated genes was investigated in QBC939 cells before and after Apr-1 transfection using the cell cycle gene microarrays. 
Results:Apr-1 mRNA was expressed in QBC939 cells transfected with Apr-1 gene, suggesting successful establishment of the QBC939 cell model stably expressing Apr-1 gene. The cell cycle gene microarrays demonstrated that the expression of Skp2 and UBE1 was up-regulated, while the expression of MRE11A、CKS2、CDK8 and CDC45 was down-regulated by more than 3 folds. 
Conclusions:The cell cycle gene microarray analysis revealed multiple alterations of the cell cycle-associated genes that were regulated by Apr-1 gene, which may help further understand the mechanism by which Apr-1 gene contributes to the regulation of cell cycle.