Objective:To observe the effects of galectin-3 on proliferation of endothelial cells induced from bone marrow mesenchymal stem cells.
Methods:The cultured mesenchymal stem cells in vitro were isolated and purified from Sprague-Dawley rats bone marrow by gradient centrifugation on lymphocytes separation medium. Cells of passage 3 were differentiated into endothelial cells by vascular endothelial growth factor(VEGF)and basic fibroblast growth facotr(bFGF).These cells were identified as endothelial cells by immunohistochemistry stain and electronic microscopy 14 days after induction.Then, the cells were cultivated with the galectin-3 for 24 hours with centrations of 0.1μg/mL，1μg/mL and 5μg/mL.The proliferation of endothelial cells induced from bone marrow mesenchymal stem cells was measured by MTT, and flow cytometry was applied to observe cell cycle.
Results:(1)Moderate and high concentrations(1μg/mL and 5μg/mL) of galectin-3 promoted proliferation of endothelial cells (P<0.05);(2) Flow cytometry showed that S phase cells increased obviously in every group (P<0.05)and G2M phase increased in the concentrations of 1μg/mL and 5μg/mL(P<0.05).
Conclusions:Galectin-3 can promote the proliferation of endothelial cells induced from bone marrow mesenchymal stem cells.