Objective：To determine the inhibitory effect of synthetic KLK12 short interfering RNA on the expression of KLK12 gene in human gastric cancer line MKN-45 cells, and to elucidate its effect on proliferation of MKN-45 cells in vitro.
Methods：The constructed KLK12-siRNA plasmid was stably transfected into MKN-45 cells by lipofectamine，then, MKN-45 cells were divided into negative control group，blank control group and KLK12 siRNA group. KLK12 expression was determined by real time PCR and Western blot. The proliferation，migration and invasion of transfected MKN-45 cells were evaluated by MTT assay，cell Migration and Invasion Assay Combo Kit.
Results：KLK12 siRNA significantly inhibited the expression of KLK12 in gastric cancer cells at both mRNA and protein level. The proliferation of MKN-45 cells in KLK12 siRNA group was significantly suppressed and the ability of migration was also significantly decreased in vitro.
Conclusions：The KLK12-siRNA expression vector can effectively suppress the proliferation and migration of MKN-45 cells，which may provide a novel gene therapy for gastric cancer.