Objective：To stdy the differential protein expression influensed by RASSF1A gene in human gastric cancer cell line SGC7901.
Methods：The proteins of SGC7901 and RASSFIA-SGC7901 were isolated by two-dimension gel electrophoresis (2-DE). Two-DE map was established by Coomassie brilliant blue staining. The well differentially expressed protein spots were screened by Image Master imaging. The specific protein spots were identified by peptide mass fingerprints using matrix-assisted laser desorption/time of flight mass spectrometry (MALDI/TOF/MS)and through searching database using Mascot software.
Results：Eight differential protein spots were screened and identified. They included galectin-1,TRP-14,ACBP,PSMB5,PSMB4,TIM,vimentin and CD79α. Overexpression of RASSF1A up-regulated the mRNA expression of galectin-1, TRP-14 and ABCP in SGC7901.
Conclusions：Galectin-1，TRP-14 and ABCP may be involved in growth inhibition induced by RASSF1A in SGC7901.