Objective：To study the changes of NF-κB expression in kidney tissues and the protective effect of xuebijing injection on ischemic-reperfusion (I/R) of hind leg of rats. 
Methods：The I/R models were prepared on thirty Wistar male rats, and were divided into  five groups: A,control group;B, ischemia only group; C, ischemia- reperfusion group; D, I/R with low dosage xuebijing injection group；E, I/R with high dosage xuebijing injection group. Serum level of CK and content of MDA in kidney tissues of each group were examined.At the same time,the expression of NF-κB in kidney tissue was detected with real-time polymerase chain reaction and immunohistochemical technique,respectively.
Results：Compared with control group, the expression level of NF-κB increased in ischemia group and significantly increased in I/R group（P<0.01）; in two xuebijing treated groups, the expression level of  NF-κB decreased，but there was no difference between groups D and E（P>0.05）. Compared with control group,  serum level of CK and kidney tissues content of MDA were increased in ischemia group and I/R group（P<0.01）. Compared with I/R group,serum level of CK and kidney tissues content of MDA were decreased in xuebijing treated group（P<0.01）.
Conclusions：The expression of NF-κB in kidney tissue is significantly upregulated in limbs of I/R model rats. The xuebijing injection has protective effect on kidney injury against skeletal musle ischemia-reperfusion injury and the protective effect is not dosage related.