Objective：To study the effect of LY294002, an inhibitor of PI3-Kinase, on human cholangiocarcinoma QBC939 cells.
Methods：The cell proliferation was detected by MTT assays. Apoptosis was analyzed by flow cytometry. The phosphorylation of Akt and expression of caspase9 were assessed by Western-blotting.
Results：LY294002 inhibited the proliferation and induced apoptosis in human cholangiocarcinoma QBC939 cells in a dose-dependent manner. Furthermore, Western-blotting showed that LY294002 attenuated the phosphorylatioin of Akt and up-regulated the expression of caspase3, 9.
Conclusions：LY294002 up-regulates the expression of caspase3, 9 by inhibition of the activity of PI3-K/Akt signaling pathway, induction of apoptosis and decreasing  cell proliferation.